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Two patients were excluded from the multivariate analysis due to missing data in PaO2/FiO2 and vasopressor use.
Duration of hypoglycemia was a significant predictor of outcome in univariate analysis, but was not included in the multivariate analysis due to several missing data.
Although SOFA score was strongly associated with mortality in the univariate analysis (Table 2), it was not included in the multivariate analysis due to small case number with SOFA score on day 0 available in our prospective observational study design.
Hence, it cannot be excluded that an effect from e.g. low physical activity and/or low fruit and vegetable intake as found in the crude analysis would be hidden in a multivariate analysis due to co-variation to alcohol and smoking.
Again the TT/SP doses variable was dropped from the multivariate analysis due to multicollinearity.
Pathological staging was excluded from multivariate analysis due to potential interaction with tumor size.
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However, these associations did not remain significant in the multivariate analysis, possibly due to the limited number of included cases.
In our study, in vitro resistance to Ara-C seemed to be associated with low probability of survival, although this did not achieve statistical significance in multivariate analysis, possibly due to the limited sample size.
We believe that the lack of statistical significance in some of the trends observed on multivariate analysis was due to a lack of study power to detect these differences.
In our cohort recent surgery, ICU admission and central line placement were all associated with higher infection rates on bivariate analysis; however, these were not significant independent risk factors on multivariate analysis possibly due to the relatively small number of actual infections documented.
Although stage IV itself was also a significant poor prognostic factor for survival in PBL patients by univariate analysis, it failed to show independent prognostic significance in multivariate analysis, probably due to the strong correlation between stage IV and multifocal bone involvement.
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