Recently, two PCR-based typing methods have come into view: multispacer sequence typing (MST) [ 17] and multi-locus variable-number tandem repeat analysis (MLVA) [ 18, 19].
According to multispacer sequence typing (8 ), this C. burnetii isolate was a new genotype, MST51.
Multiple genetic methods have been developed to identify different strains of C. burnetii, including restriction fragment length polymorphism (RFLP) [ 18], multilocus variability analysis (MLVA) [ 19, 20], and multispacer sequence typing (MST) [ 21].
We used a more stable, sequence-based typing method, multispacer sequence typing (MST), on samples from humans and a group of ruminant animals (goats, sheep, and cattle) to establish a firmer correlation between Q fever cases in humans and animals (7 ).
We investigated Multispacer Sequence Typing (MST) for M. tuberculosis genotyping.
Multispacer sequence typing (MST) genotyping is based on the sequencing of several intergenic regions selected after complete genome sequence analysis.
*MST, multispacer sequence typing; Ct, cycle threshold; BAL, bronchoalveolar lavage; NA, not applicable.
Multispacer sequence typing is the first reliable method for typing Coxiella burnetii isolates.
Multilocus sequencing analysis [ 23] and multispacer sequence typing [ 24] differentiated M. massiliense from M. bolletii.
MLVA-6 and multispacer sequence typing results were consistent with the MLVA-12 results.
