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Prior to addition, InlB-coated beads were briefly sonicated, diluted in DMEM, and added to siRNA-treated HeLa cells at a multiplicity of approximately 100 beads per cell.
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Using an infection multiplicity of 3PFU/cell, approximately 20% of CD11c+ DCs were BAC-eGFP+ after 18 h and approximately 30% of CD11c+ cells were gM-eGFP+.
Inoculum was diluted in 1 ml medium containing a multiplicity of infection approximately of 8 Tcell0/adherent cell DENV-1.
Arf−/− HCC cells were infected with green fluorescence protein (GFP) or FoxM1-expressing adenovirus for 48 or 72 h at a multiplicity of infection approximately 500 pfu/cell.
Confluent monolayers of Vero cells were infected with IBV at a multiplicity of infection of approximately 2 PFU/cell.
One ml of the respective suspensions containing 108 spirochete cells was added per well, to give a multiplicity of infection of approximately 100.
Confluent monolayers of Vero cells in six-well plates were infected with IBV at a multiplicity of infection of approximately 3 PFU/cell.
Phage stocks in either in LB or TM buffer were combined with the cells on ice for 15 minutes at the multiplicity of infection of approximately 10.
At 16 hours post-transfection, the cells were infected with IBV at a multiplicity of infection of approximately 0.5 PFU/cell, and were harvested at 24 hours post-infection.
Subconfluent monolayers of C6/36 cells were infected at a multiplicity of infection of approximately 0.03 focus-forming units (ffu /cell or mock infected.
Cells were infected at a multiplicity of infection of approximately five and selected in medium containing 0.6 μg/ml puromycin for at least three days before use.
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