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Incubation of the young leaves on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (0.5 mg/l) and indole-3-acetic acid (0.1 mg/l) resulted in the best multiplication rate for organogenesis.
It was not possible to determine the multiplication rate for the adherent 293T cells due to their tendency to grow up the walls making them difficult to count.
The multiplication rate for each clone was obtained by calculating the fold change in parasitemia (parasitemianew/parasitemiastart) for each 48 hour cycle.
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Subsequently, parasites were grown in the respective RBC cultures for four complete 48-h cycles, showing a mean multiplication rate of 4.9±0.3 per RBC cycle for the GR-deficient RBCs, as well as 4.3 (0+ blood) and 6.5 (A+ blood) for the controls (mean 5.4±0.5; data are given in Table 1).
For instance, several but not all studies have noted a reduced multiplication rate of P. falciparum in hemoglobinopathic erythrocytes (Friedman, 1978; Glushakova et al., 2014; Pasvol et al., 1978).
During asexual blood stage cycles of malaria parasites, there is high load on protein synthesis and turnover because of rapid multiplication rate of the parasite; therefore, proper functioning of protein quality control machinery is essential for maintenance of cellular homeostasis.
The suitability of culture media for large-scale production of Rhizobium depends upon the utilization of carbon source and multiplication rate of bacteria.
The MIC is the blood or plasma concentration giving a parasite multiplication rate of 1.
From this finding it was suggested that the difference accounts for the higher virulence of strain OY-W due to a depletion of sugars in the phloem sap and the resulting higher multiplication rate of the phytoplasmas [ 49].
By convention, all participants with fewer than four qPCR positive measurements (N = 8), were excluded from multiplication rate analysis for a total of 13 evaluable participants.
Somatic embryogenesis has long been considered a superior propagation system for many crops [ 53- 55] because of its inherent high multiplication rate and potential for year round, uniform disease free plant production.
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