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PTEN IHC was scored as the multiplication of intensity and proportion and ranged from 0 to 300 in cytoplasmic staining.
The H-score was obtained by multiplication of intensity with percentage (range 0 to 300), summed up for the two cores and divided by two.
Multiplication of intensity and percentage scores (staining index) was utilized to determine the following results: 0, 1, 2, 3, 4, 6, and 9.
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Also, the intensity of the fluorescent transmittance onto the p-FET is linearly proportional to the multiplication of the intensity of emitted fluorescence (Pem) from fluorochrome before transmission and the transmittance coefficient (T) of the optical filter for the emitted wavelength: P FET = T P em.
The IRS is obtained by multiplication of staining intensity by the number of stained cells.
Staining index was calculated as the multiplication of staining intensity score and the proportion of DUSP-9-positive tumor cells.
The final immunohistochemical score (IHS; ranging from 0 to 12) was obtained by multiplication of the intensity score and the quantity score.
Multiplication of the intensity and the percentage scores gave rise to the final staining score: 0 (negative), + (1-4), ++ (5-8), and +++ (9-12).
The IRS for each individual case ranging from 0 to 12 was calculated by multiplication of the intensity and frequency scores.
The final immunohistochemical score (ranging from 0 to 300) is obtained by multiplication of the intensity score and the frequency score.
Multiplication of the field intensity enhancement with the QE enhancement gives the total fluorescence enhancement (Fig. 5d).
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