Sentence examples for multiplication medium from inspiring English sources

The germinated embryos were transferred to a banana multiplication medium for generating multiple shoots of each transformation event (Fig.  2E, G and I).

> -wrap-foot> Aftexcisinging multiple shoots, when mother explants were transferred to the fresh shoot multiplication medium (MS medium + 1.0 mg L−1 BAP), they proliferated significantly during the next two subcultures and reduced thereafter (Fig. 2).

As a shoot multiplication medium which contains charcoal, LPch is generally used for between 28 42 days; growth on this medium would be dependent on the microorganisms present.

The bacterial mass was then transferred to the multiplication medium (sugarcane juice or molasses) and incubated at 35 °C, with agitation, till an optical density (540 nm) of about 0.5 was achieved.

The four standard tissue culture media were: shoot initiation medium ½LP5 (Hargreaves and Menzies 2007), shoot multiplication medium LPch (Hargreaves and Menzies 2007), embryogenesis medium EDM6 (Hargreaves et al. 2009) and embryogenesis medium Glitz (Hargreaves et al. 2009).

Yeast inoculum was prepared by separately inoculating two loops of each yeast strain in 125-mL flasks containing 50 mL of the multiplication medium (clarified sugar cane juice or molasses with approximately 4 g 100 mL−1 of total reducing sugars, pH 5.5, as supplied by a local fuel ethanol-producing unit, and sterilized at 120 °C for 20 min).

The successful in vitro multiplication of potatoes depends on the presence of a suitable combination of auxins with gibberellic acid (GA3) in the propagation medium [ 4– 7].

After this period, the calli were transferred to a shoot differentiation and protocorm - like body (PLB) multiplication and elongation medium (A10 medium) with A4 medium as control.

In the same way as the experiment of shoot differentiation and multiplication on A10 medium with CA10 calli, organogenic calli were cultured on A4 medium (CA4 calli) as control.

Both plasmids were individually transformed into E. coli JM109 competent cells for multiplication on SOB medium with Amp (100 μg/mL) at 37 °C for 24 h.

At the second day of treatment, at 0.5% DMSO in culture medium, cell multiplication was increased significantly whereby at 2% a significant attenuation was observed (Fig. 11A).