The cytokine concentrations of IFN-γ, IL-5, IL-6, IL-8, IL-10, MIP-1β, MCP-1, and MIP1-α in plasma as well as in cultured supernatants were determined using a multiplex immunoassay using bead array technology.
Relative quantification was performed in a multiplex reaction using β-actin as endogenous control.
The receivers using the multiplex scheme using the random algorithm will receive the complex interference, which decreases the multiplex gain.
SNPs in multiplexes were amplified using Qiagen Multiplex PCR kit (Qiagen, Hilden, Germany) and further analysed with single base extension (SBE) reactions using SNaPshot Multiplex Kit (Applied Biosystems, Foster City, CA).
The same outer PCR was used as template both for 10 separate inner PCRs for Pyrosequencing as well as an inner multiplex PCR, used for PrASE.
Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems.
Cong, L. et al. Multiplex genome engineering using CRISPR/Cas system.
Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster.
Title Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster.
Since Cas9-based multiplexing has successfully been performed in other mammalian cells (Cong et al., 2013; Wang et al., 2013), multiplexing using the Cas9 system may as well be a powerful technique in CHO cells.
