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Multiplex tests are being developed which allow for the simultaneous detection of multiple pathogens.
Some techniques are difficult to adapt to the emergency context (multiplex tests, ELISA or high-flux cytometry).
Current trends are to use a combination of biomarkers notably cytokines with multiplex tests providing simultaneous measurements of several biomarkers from a single biological sample.
Assays used often are not standardised (especially for ELISA and "multiplex" tests), making it difficult to compare results from different studies.
Nevertheless, persisting difficulties arise when interpreting measurements of biomarker levels, a problem that is compounded by the dissemination of multiplex tests [6], thus increasing the volume of information generated.
Today, several multiplex tests are commercially available [ 9- 16].
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Lyme Multiplex testing is recommended after that time.
Description: The multiplex test is based on fluorescent bead technology that allows the simultaneous measurement of antibodies to different B. burgdorferi antigens in a single sample.
The first strategy employs a 13 STR loci multiplex incorporating three mini-STRs into the current multiplex test.
The multiplex test design should be applicable to other ascomycete species, of use in studies of MAT distribution and facilitating sexual crossing by identifying compatible isolates.
The conclusion was that "… a substantial number of existing patents on genes and methods of diagnosis pose a threat to the development of multiplex testing, parallel sequencing, and whole-genome sequencing, the areas of genetic testing with the greatest potential future benefits".
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