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Multiplex testing for ANA and ANCA (anti-neutrophil cytoplasmic antibodies) specificities is expanding.
A viral co-infection rate >30% supports the use of multiplex testing for viral and bacterial pathogens in children with respiratory infections of uncertain etiology.
In addition, multiplex testing for driver mutations in 72 squamous cell carcinomas of the lung detected: PIK3CA mutations in 8%, PTEN mutation/deletion in 28%, FGFR1 amplification in 26%, and unknown genetic alterations in 39% [ 9].
A new assay, termed Septi Fast (Roche Diagnostics, Mannheim, Germany), enables rapid, multiplex testing for micro-organisms using a real-time polymerase chain reaction that is coupled to melting curve analysis.
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The recovered c-DNA was additionally multiplex tested for three EMT markers [Twist1, Akt2, PI3Kα] and separately for the tumor stem-cell markers ALDH1.
The recovered c-DNA was additionally multiplex tested for three EMT markers [TWIST1, Akt2, phosphoinositide kinase-3 (PI3Kα)] and separately for the tumor stem cell marker ALDH1.
To overcome limitations concerning the use of several monoplex tests in parallel and the resulting shortage of sample volume, the development of multiplex tests for a fast and exact identification is necessary.
The demand for faster multiplex testing is high and new techniques are emerging.
Multiplex testing is a practical approach for detection of the most relevant targets of interest in a single reaction with minimal sample input and shorter turn-around time.
Other multiplex testing provides the additional testing information without the need for specific target selection (e.g., DNA sequencing).
Respiratory samples were analysed either with a rapid RSV test if the patient had symptoms suggestive of bronchiolitis, followed by multiplex PCR testing for ten respiratory viruses, or with multiplex PCR testing alone if the patient had suspected other ARI.
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