Sentence examples for multiplex step from inspiring English sources

This was to avoid amplification of the short overlapping fragments during the multiplex step.

In the first multiplex step, the four primer pairs were divided into two sets, A and B (Table 3).

The PCR products of the first step were diluted 20-fold in water and 5 µl were used as templates in second step singleplex reactions using the same primer pairs as in the multiplex step.

Primer sequences and PCR conditions for the multiplex step were as described by Fackler and colleagues [ 25].

In the first multiplex step, 150 nM of each primer pair and 5 μl of DNA extract were added to a final reaction volume of 20 μl.

For the cave bear a nested primer design was chosen where the primers in the singleplex amplification are shifted inwards compared to the primers used in the multiplex step.

The multiplex PCR step provides mass amplification using common primers, which increased markedly the sensitivity of the test.

The system consisting of a multiplex PCR step followed by macroarray allowed us to detect multiple target bacterial species simultaneously without the use of agarose gel electrophoresis.

The final multiplex PCR step of the method may be applied if identification of the serogroup, biotype and/or virulence factor level is necessary.

A multiplex preamplification step was used to increase the quantity of specific cDNA target (TaqMan Pre-Amp Master mix Kit, Applied Biosystems) following manufacturer's instructions.

As MOL-PCR includes a multiplex ligation step, followed by a singleplex PCR and analysis with microspheres on a Luminex device, several parameters are accessible for optimisation.