Sequential multiplex staining procedures were performed according to standard protocols using a combination of fluorescence-conjugated secondary antibodies and the tyramide signal amplification (TSA) method [15].
In IHC, multiplex staining is growing need within limited quantity of clinical samples.
Later strategies incorporated multiplex staining of surface markers and cell sorting to exclude cell types other than HSC from cell purifications.
In this work we have successfully used a multiplex staining approach to deeply characterize the proteome and phospho-proteome of grapevine in response to phytoplasma infection by 2-DE.
In this work, we used a multiplex staining approach with 2D gels to study quantitative changes in the proteome and phosphoproteome of Flavescence dorée-affected and recovered 'Barbera' grapevines, compared to healthy plants.
Using a multiplex staining approach on the same gel, we were able to overlap the images and precisely match them together, in order to normalize the data and distinguish a lightly phosphorylated, high abundance protein from a heavily phosphorylated, low abundance protein.
