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In addition to fluorescent real-time PCR, some methods have also been described where amplified products are labeled and hybridized to arrays [ 7- 10] and multiplex setups have been suggested [ 11, 12].
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Subsequently, DNA of the corresponding pools was converted into standard fragment libraries and sequenced in multiplex setup using barcodes that were introduced during library preparation.
We performed a multiplex PCR setup [ 72] to amplify all three fragments at once in a separate Class II Safety Cabinet (Thermo Scientific) in the dedicated clean-laboratory using the "Type-it Microsatellite PCR Kit" (Qiagen, Hilden, Germany).
These loci were selected for setup of multiplex STR assays and utilized in genotyping of larger sample cohort.
A potential way around such limitations is provided by fiber-integrated setups exploiting time-multiplexing approaches.
Amplification tests led to the setup of two multiplex PCR mixes allowing the amplification of 21 loci.
With this technique we multiplex the processed holograms of actual diffuse objects composing a scene using a two-beam off-axis holographic setup in a Fresnel approach.
Go to a multiplex.
These fear of the future setups make for enthralling, compact science-fiction thrillers where the fate of humanity can be conveniently decided one way or the other in the space of a two-hour trip to the multiplex.
Head to a multiplex.
Setups shrank.
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