We used eight multiplex sets of 6 or 7 microsatellite markers each (Supplementary Table S3).
The remaining 304 primer pairs, which generated unambiguous and stable PCR amplicons, were therefore selected and assembled into 41 multiplex sets of six to eight markers each (Supplementary Table S1).
The 16 markers were arranged in multiplex sets for genotyping.
The SSR primer pairs in each multiplex set were assigned one of the four different fluorescent labels (6-FAM, VIC, NED, or PET) in such a manner that no two markers with the same fluorescent dye showed overlap in allele size range.
One multiplex set built a rough map of Chr 5 (Fig. 1B), whereas the two multiplex sets for Chr 5 made up a linkage frame spanning 142.1 cM with 11 polymorphic markers (Fig. 2).
The PCR proceeded as follows: 15 min at 95 °C; 30 cycles of 30 s at 94 °C, 90 s at 53 °C, 1 min at 72 °C for multiplex set 1, or 35 cycles of 30 s at 94 °C, 90 s at 58 °C and 1 min at 72 °C for multiplex sets 2 and 3; and a final extension step of 30 min at 60 °C.
Unfortunately, one of these markers (ZF01-170) failed to amplify correctly within its multiplex set.
MPEG coder can multiplex a set of independently-coded, arbitrarily-shaped video objects and transmit it through either fixed or variable rate channels such as internet, wireless or satellite communications.
M-FISH relies on a multiplex probe set of chromosome specific "paints", each labeled with a finite number of spectrally distinct fluorophores, and a set of filters for achieving optimal color separation for all fluorophores employed.
Due to financial restrictions, we were limited to genotyping individuals in Stage 2 using a single SNPlex multiplex probe set of 47 SNPs: 23 SNPs in the social study and 24 SNPs in the nonsocial study.
