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Thus, three of these ten positions are shared for PE preparation and multiplex chemistry on HiSeq, requiring more user interaction for multiplex runs and increasing the risk of reduced reagent flow and air in the instrument tubing.
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Fragments were analysed in a multiplex run and visualised with BstYI+C and BstYI+T primers, respectively labelled with the fluorescent dyes JOE and 6-FAM.
What kind of breakout are we talking about: studio bidding wars and sell-out multiplex runs?
The adaptors had 5 nucleotides in their 3′ ends as "bar codes" for sample identification in multiplex runs.
All of the viral DNA genomic libraries, as emPCR products, were sequenced through two duplicated multiplex runs on a Roche GS FLX Titanium sequencer.
Genomic DNA was sequenced on an Illumina Hi-Seq in a multiplex run of 48 samples.
Averages of 17 million reads were obtained for each sample in a multiplex run.
The remaining isolates were sequenced on the Illumina HiSeq2500 platform by 150 bp Paired-end sequencing in a single multiplex run.
Two half-runs of sequencing for multiplex #1 (run B) and multiplex #2 (run C) were then performed by Agencourt (Beverly, MA, USA) on a GS-FLX Titanium sequencer.
After that, it'll be the conventional digital projection editions at the multiplexes, running 20 minutes shorter.
The resulting reads were de-multiplex and reported as separate runs and deposited in the National Institutes of Health (NIH) Short Read Archive (http://ncbi.nlm.nih.gov/sra) (Pancreas: SRS584523, Hypothalamus: SRS413447, Liver: SRS585609, Bone Marrow/Bursa: SRS586251).
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