Sentence examples for multiplex reactions with from inspiring English sources

The PCR consisted of 3 multiplex reactions with published primer sequences.

PCR was performed in two multiplex reactions with the QIAGEN Multiplex PCR Kit (QIAGEN, Valencia, CA, USA), each containing 30 ng of genomic DNA.

Ten neutral microsatellite loci were amplified in three multiplex reactions with annealing temperatures 54°C (reaction I and III) or 56°C (reaction II) using the QIAGEN PCR kit.

In the further development of this device, an on-chip DNA purification module will be integrated into the chip and multiplex reactions with sequence-specific fluorescent probes should be established in order to avoid the use of unspecific intercalating dyes.

Molecular genotyping of a total of 269 samples was carried out using a set of 10 microsatellite loci in three multiplex reactions, with subsequent separation of the PCR products using an ABI PRISM 3130xl DNA analyser (Applied Biosystems) with GeneScan Liz 500 as size standard (Applied Biosystems) and following the procedure of Coelho et al. [ 22].

Samples that initially showed a negative PCR reaction were repeated in both a single-plex and a multiplex reaction, with SRY as the positive control.

Each target assay was replicated three times and performed in multiplex reaction with the 18S rRNA endogenous control gene (4310893E, Applied Biosystems).

Briefly, a multiplex reaction with fluorescent-labeled primers was amplified in a multiplex polymerase chain reaction (PCR), and b-globin gene was used as a positive control for GST null genotypes.

For all markers other than sY1291 and Y-DAZ3, negative samples were repeated in a single-plex reaction and in a multiplex reaction with the SRY gene acting as a positive control.

PCR amplification was performed in one multiplex reaction with only 30 cycles of denaturing, annealing, and extension at 95°C for 30 s, 55°C for 30 s, and 72°C for 40 s, respectively.

Subsequently, the primer pairs were selected for a multiplex reaction, with one of the products including the target fragment of the studied gene and the second one as a reference.