Sentence examples for multiplex reactions and from inspiring English sources
Primer sequences for sequence analyses, multiplex reactions and extension reactions are available upon request.
In our experience, the simplex format strategy (i.e. based on testing phages and viral pathogens in distinct amplification reactions) proved to be the most simple and cost-effective for routine molecular diagnosis since it does not require the specific development of multiplex reactions and relies on a unique control reaction for DNA viruses and another for RNA viruses.
Loci that did not amplify during the first multiplex PCR were added to alternative multiplex reactions and their amplification success was tested subsequently.
32– 34 However, these studies used numerous multiplexes in a single reaction or a number of different multiplex reactions and steps to deduce the serotype.
Primer and probe sequences are shown in Table 2. Primers and probes were grouped into three multiplex reactions and one single target reaction.
Nine loci were polymorphic and were optimized into two PCR multiplex reactions and tested on 100 individuals from five riparian populations from central Belgium.
Major advantages of mRNA profiling are the possibility of detecting several body fluids in one multiplex reaction and of simultaneously isolating DNA without loss of material.
d l Double labeling of mRNAs (multiplex reaction) and splicing factors.
Microsatellite markers BAT-25, BAT-26, NR-21, NR-22, and NR-27 were amplified in multiplex reaction and analysed using DHPLC and capillary electrophoresis (CE).
Therefore we developed a novel TaqMan®-based real-time PCR assay comprising the amplification of two independent targets (MSC_0136 and MSC_1046) and an internal exogenous amplification control in a multiplex reaction and evaluated its diagnostic performance with clinical samples.
Nine single nucleotide polymorphisms (SNP), five intronic, three coding and one located in the 5' UTR, spaced out along the PREP gene were investigated using the ABI SNaPshot multiplex reaction and the ABI 3100 automated genetic analyzer.
