Sentence examples for multiplex reaction to from inspiring English sources

Moreover, we compared the expression of each gene in the multiplex reaction to its expression in a singleplex reaction.

Commercially available primers and a VIC-TAMRA-labelled probe for the housekeeping gene RNase P were included in the multiplex reaction to standardise for DNA input.

Briefly, 50 ng of genomic DNA was used in a two-step multiplex reaction to firstly amplify BRCA1 and BRCA2 coding regions followed by the incorporation of molecular barcodes (multiple identifiers, MIDs) and 454 adapters to each amplicon.

Amplification was performed in three different multiplex reactions to avoid overlapping fragments (Supplementary Table 3).

The above data demonstrate that the concentration of Taq polymerase needs to be optimized based both on the number and the sequences of the primers added to a multiplex reaction, in order to obtain maximal efficiency without mis-priming.

0.5 1.0 µl of cDNA was used as template in a multiplex PCR reaction to compare the expression of cuti-1 with a control gene ama-1, which encodes RNA polymerase II [20], and sqt-1, which encodes a cuticle collagen [8].

It is rather common for a multiplex PCR reaction to yield bands with unequal brightness.

Primers were labeled with fluorescent markers to distinguish between fragments of similar size, and microsatellite markers were grouped in multiplex reactions, according to PCR conditions and expected fragment sizes [ 6].

Standard PCR was used for fragments that failed amplification in the multiplex reactions and to amplify the HVR1 of some additional samples.

On the other hand, for PCR typing, multiplex reactions required to identify all the serotypes were added.

J1-M267 Y chromosomes were analysed for a panel of eight Y-chromosome microsatellite loci (DYS19, YCAIIa/b, DYS389I/II, DYS390, DYS391 and DYS392) by using multiplex reactions according to the STR DNA Internet Data Base [ 20] and fragment analysis by capillary electrophoresis on ABI PRISM® 3100 Genetic Analyzer.