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Multiplex quantification of cytokines in all samples was performed using the Luminex R 100 System (Luminex, Austin, TX, USA).
Another group reported a multiplex quantification of miRNAs involving the purification of multiplex PCR amplicons followed by uniplex analysis on microfluidic chips [ 15].
Measurements performed on plasma were established biomarkers (n = 47), lipidome by LC-MS (n = 108), metabolome by GCMS (145) and multiplex quantification of 79 plasma proteins.
Investigators trying to develop assays for the accurate multiplex quantification of gene expression in small samples face many technical hurdles [ 5].
TRAC has been applied for gene expression analysis of microbial cultures [ 12], multiplex quantification of bacterial populations [ 14, 15] and to monitor gene expression stability in continuous cultures [ 16].
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In the last years, MRMmultiplex assay have become very popular due to their generic concept and theability to generate multiplex quantifications.
Clearly for any multiplex sequencing protocol, accurate quantification of high molecular weight DNA remains a procedural bottleneck, and is the most likely source of sample-to-sample variation in sequence coverage.
Multiplex amplicon quantification consists of a multiplex polymerase chain reaction amplification of fluorescently labelled target and control amplicons, followed by fragment analysis on an ABI3730 DNA Analyser (Applied Biosystems).
Next we evaluated the use of high multiplex amplicon barcoding in targeted quantification of RNA transcripts.
The ability to multiplex the analytes allows effective quantification of protein panels across multiple samples by monitoring specific transitions (precursor-fragment ion pairs) during a predefined elution window.
Recently, a multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells has been published [ 23] using TaqMan probes and an Epstein-Barr virus (EBV) standard curve.
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