Sentence examples for multiplex qPCR for from inspiring English sources

A TaqMan based multiplex qPCR for B. anthracis was performed.

The present study describes the development and clinical validation of a multiplex qPCR for the molecular diagnosis of BV.

We used multiplex qPCR for four mouse mtDNA genes (16S rRNA, CO1, CO3, CytB) normalized to the geometric means of three abundantly expressed housekeeping genes (GAPDH, beta-actin, 18S rRNA) in each sample to improve precision of our assays.

Subsequently, the multiplex qPCR assay for molecular diagnosis of BV (BV-PCR) was compared with the NS as reference method for the diagnosis of BV.

While validating the specificity of a multiplex qPCR assay for the detection of Y. pestis (6 ), we obtained DNA from the dissected peritoneum of a black laboratory rat (Rattus rattus), which tested positive for the pla gene.

The present study describes the development of a multiplex qPCR assay for the semi-quantitative detection of well-recognized BV-associated marker organisms and different Lactobacillus species and its evaluation in a clinical setting.

Stool samples from 45 travellers travelling in Benin, West Africa, were analyzed by a new multiplex qPCR assay for Salmonella, Yersinia, Campylobacter, Vibrio cholerae, Shigella or enteroinvasive (EIEC), enterohaemorrhagic (EHEC), enterotoxigenic (ETEC), enteroaggregative (EAEC), and enteropathogenic Escherichia coli (EPEC).

Primers and TaqMan probes used for multiplex qPCR assays, or primer pairs for SybrGreen assays, were designed using Beacon Designer software, and the qPCR assays were performed on a CFX 96 Real Time System (C1000 Thermal Cycler, BioRad).

The presence of bacterial DNA in the sample was first screened by a TaqMan-based multiplex qPCR using universal primers for the 16S rDNA gene for differentiation between Gram-positive and Gram-negative bacteria.

All samples were screened by specific Gram probes for multiplex qPCR.

Equal amounts of RNA (1 μg) were reverse-transcripted to obtain the cDNA for multiplex QPCR with specific probes (see Additional file 1).