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Initially, these multiplex protocols were tested using different serial dilutions of genomic DNA extracted from peripheral blood (from 100 ng/mL to 5 pg/mL).
Thermal cycling profiles for the multiplex protocols were as follows: 15 min at 95°C, followed by 36 cycles of 30 s at 94°C, 90 s at 58 61°C and 60 s at 72°C, followed by final extension step of 10 min at 72°C.
Thermal cycling profiles for the multiplex protocols were as follows: 15 min at 95°C, followed by 36 cycles of 30 s at 94°C, 90 s at 58°C and 60 s at 72°C, followed by final extension step of 10 min at 72°C.
Similar(57)
All the faecal samples identified by a mDNA-based PCR-RFLP method as pine marten were genotyped at 15 microsatellite loci using a multiplex protocol.
Following DNA extraction, we used the PCR multiplex protocol of Chong et al. (2000) [54] to detect the presence of the α-globin 3.7 deletion.
Genotyping was performed using a multiplex protocol (see Supporting Information).
Microsatellite genotyping was conducted using a published multiplex protocol [ 39].
A multiplex protocol was used for L10, CG49 and CG108 (Taris et al. 2005).
The Illumina multiplex protocol for DNA introduces the barcode (or "index") to the library in the adapter oligo.
An average of 5/6 pups per breeding pair were kept in separate cages and genotyped using a Multiplex Protocol for Human SOD Taconic USS).
Ponderosae (Fig. 1; Appendix 2) using a PCR multiplex protocol that integrates fluorescent labels during PCR (Culley et al., 2008; Culley et al., 2013).
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