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The SNPs were genotyped by using primer extension of multiplex products with detection by matrix-assisted laser desorption ionization – time of flight mass spectroscopy using a MassARRAY Compact Analyzer (Sequenom, San Diego, CA, USA).
Multiplex products (1 : 140 dilutions) were genotyped using an ABI 3730 sequencer.
For the fragment analyses, 1 μL of 1 20 diluted multiplex products was used.
All genotyping was done using the primer extension of multiplex products with detecting by matrix-assisted laser desorption ionization time of flight mass spectroscopy using a MassARRAYCompact Analyzer (Sequenom, San Diego, CA, USA).
All genotyping was done using the primer extension of multiplex products with detecting by matrix-assisted laser desorption ionization-time of flight mass spectroscopy using a MassARRAY Compact Analyzer (Sequenom, San Diego, CA, USA).
For de novo replication in stage 2, all selected SNPs were genotyped in the Hong Kong and Shanghai case control samples by a primer extension of multiplex products with detection by Matrix-assisted laser desorption ionisation-time of flight mass spectroscopy using a MassARRAY platform (Sequenom; San Diego, CA, USA).
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(I'd gladly take the bracingly crazy "Exorcist II" over most of the past year's machine-tooled multiplex product).
The population was genotyped by primer extension of multiplex PCR products with the detection of the allele-specific extension products by matrix-associated laser desorption/ionization time of flight (MALDI-TOF; Sequenom MassARRAY, San Diego, CA).
Next, multiplex SBE products from different panel types were pooled prior to the hybridization step.
Multiplex PCR products were easily identified in agarose gels with no background noise.
The proposed method was applied to simultaneously detect the multiplex PCR products of four foodborne pathogenic bacteria under the optimal conditions within 8 min.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com