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Multiplex optimisation was verified by consistency with singleplex reactions.
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This optimisation was undertaken outside KNIME.
Parameter optimisation was carried out by OEA and PEB.
The relevance of gemcitabine timing for chronotherapeutic optimisation was investigated.
For example, extensive optimisation is typically required for multiplex SSR amplification in bread wheat [ 12], one of the world's most important cereal crops.
Could social media optimisation be the answer?
After all, SEO (search engine optimisation) is all about content.
When it is nonlinear, numerical optimisation is necessary.
However, since there is a cost involved, optimisation is required.
But the art of SEO (search engine optimisation) is often neglected.
The annealing temperature used for each microsatellite after optimisation is given in Supporting Information Table S6.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com