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The Legend Of Hercules Whenever two seemingly identical movies go head-to-head at the multiplex, one must inevitably lose.
As well, the monoplex PCR format is being replaced by a multiplex one.
Nucleus" shown on the predicted result window (Fig. 3c), meaning that the protein is a multiplex one, which can simultaneously occur in "cytoplasm" organelle and "nucleus" organelle, fully consistent with experimental observations.
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Real-time reverse transcription PCR (rRT-PCR) was performed on nucleic acid extracts by using the Path-ID Multiplex One-Step RT-PCR Kit (Life Technologies) according to the manufacturer's recommendations.
This multiplex one-step RT-PCR assay was evaluated using 376 samples collected during the year 2003.
In this study, we developed a novel, rapid and efficient antigen-specific TCR-cloning system by combining three technologies: multiplex one-step RT-PCR, transcriptionally active PCR (TAP) and luciferase reporter assays.
Multiplex one-step RT-PCR with leader primers designed from leader peptide sequences of TCRs enabled us to amplify cDNAs of TCRα and β pairs from single T-cells with remarkably high efficiency.
A multiplex one-step RT-PCR assay was designed to simultaneously amplify the HA and NA genes of the four subtypes of influenza A viruses and NS genes to discriminate type A and B viruses.
We applied multiplex one-step qRT-PCR to evaluate mRNA expression levels of all four Tensins in this clinical study of total RNA extracted from 223 RCC and 48 normal kidney cortex tissue samples.
Conditions for one-step RT-PCR were similar to those for multiplex one-step RT-PCR.
RNA was extracted from these samples and a multiplex one-step real-time RT-PCR assay was developed to specifically detect the influenza A virus (FluA).
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