Dan Salem, PhD candidate in the Department of Chemical Engineering and member of the Strano research group, explains a project funded in 2015 by a J-WAFS Solutions grant: A Multiplex, Nanosensor Platform for the Real Time Monitoring of Food and Water-Borne Contaminants.
The developed NIR PKA and JNK biosensors enable multiplexing with blue-green optogenetic tools for probing and monitoring of multiple cell processes for better understanding of mechanisms that mediate regulation and specificity of PKA and JNK kinases.
The use of a fluorescent probe enables monitoring of reactions in real-time and multiplexing capabilities.
In order to improve monitoring of the rapidly expanding worldwide TYLCD epidemic, a multiplex polymerase chain reaction assay (mPCR) was developed.
By multiplexing various sensing elements, the contact lens sensors would ultimately enable wireless, continuous and noninvasive monitoring of physiological conditions, as well as the detection of biomarkers associated with ocular and other diseases.
The development of a non-invasive multiplex molecular imaging technique capable of high resolution at significant tissue depths would aid in the diagnosis and monitoring of diseases such as cancer.
Greater monitoring of standards.
For the monitoring of humoral immune responses, peptide-specific IgGs reactive to each of the 31 different peptides, including both vaccinated and non-vaccinated peptides, were measured by bead-based multiplex assay.
Finally, we took advantage of the spectrum of known fluorescent proteins to generate green, yellow, cyan, and red reporters, paving the way for multiplex protease monitoring.
There are distinct advantages of the FMIA over the ELISA, which include higher sensitivity, higher sample throughput analysis, and the ability to multiplex and monitor exposure to multiple pathogens simultaneously in a single sample.
