Sentence examples for multiplex mix was from inspiring English sources

When genotyping the whole pedigree, a Qiagen multiplex mix was used during PCR amplification.

Due to the convenience of the presence of the passive reference ROX in the QuantiTect Multiplex buffer, which was not in the EpiTect Methylight mix for multiplexing on a ABI7500, the QuantiTect Multiplex mix was used in subsequent optimization experiments.

To evaluate the performance of the PRI-lock system in a multiplex setting, the probe mix was ligated on several target DNA mixtures and analyzed in conventional real-time PCR.

For this purpose, the EpiTect MethyLight Mix (Qiagen), the iQ Multiplex Powermix (Bio-Rad), the Genotyping Master Mix (Applied Biosystems) and the QuantiTect Multiplex Mix (Qiagen) were tested on 50 ng of bisulfite treated SiHa DNA, which is positive for CADM1, MAL and hsa-miR-124-2 hsa-miR-124-2 hsa-miR-124-2 hsa-miR-124-2

Following the manufacturers instructions for each multiplex RT reaction a TaqMan master mix was prepared using Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA); a final volume of 100 µL/port (which supplies the master mix to 48 individual wells) was loaded into each of the 8 ports/TLDA card.

Thirty microliters of virus-specific master mix was prepared containing 15 µl of 2x QuantiTect Multiplex PCR NoROX Master mix (contains HotStarTaq DNA polymerase, Qiagen), 500 nM of each primer and 100 nM probe.

The reaction mix was prepared according to the microsatellite amplification procedure in the QIAGEN® Multiplex PCR Handbook and 0.5 ng of DNA.

Multiplex primer mixes were made by adding equal amounts of all primers in that set at a concentration of 100 µM, and then adding either 4.0 µl (Amplification Sets 1 and 3) or 4.4 µl (Amplification Set 2) to each 20 µl 1st-round PCR tube, to give a final concentration of 1 µM.

Four multiplex mixes have been developed.

Furthermore, the HotStarTaq polymerase present in the QuantiTect Multiplex PCR buffer in the PCR master mix is inactive at 30°C.

The Genotyping Master Mix gave the highest Cq values combined with the highest ΔRn values, indicating that this master mix is not ideal for multiplex qMSP (Table 1).