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Our results demonstrate that this multiplex microarray is an effective and versatile tool to detect transcriptome responses at high sensitivity.
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The NimbleGen Human DNA Methylation 3x720k CpG Island Plus RefSeq Promoter Microarray is a multiplex slide with 3 identical arrays per slide.
Multiplex PCR and microarray were employed for molecular detection of serotypes in cocolonized samples.
Performance characteristics of the diagnostic test are shown in Table 3. Positive predictive values associated with multiplex PCR and microarray were greater than 90%; the negative predictive value and accuracy rates were greater than 95%.
The analytical sensitivities of the multiplex PCR and DNA microarray were higher than 80%, and the analytical specificity was nearly 100% for all tests.
However, thanks to their decreasing cost, the development of syndrome-based multiplex PCR assays or microarrays is likely to increase in the coming years.
Application of biochip for concurrent detection of various immobilized target DNA and protein molecules and multiplex of DNA and protein on the same microarray was accomplished.
Once a calibration curve was established with varying dilutions of each clone, 24 mtDNA samples from DLPFC that showed varying levels of heteroplasmy with microarray were run using the SNaPshot Multiplex Kit.
In addition, none of the NAATs were run 24/7; multiplex PCR was performed once daily whereas the microarray was ran every second day.
A custom designed microarray was manufactured on the Roche NimbleGen (Madison, WI) multiplex (12-plex) long-oligonucleotide (60 nt) platform.
In this study, a new Fusarium graminearum microarray was designed for the 8 × 15 k Agilent multiplex format.
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