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Effective multiplex methods for GMO detection and identification are being developed to reduce time and associated costs of analysis.
Applying novel 96-well format multiplex methods for Illumina library preparation (NEXTERA®) and a personal sequencer (MiSeq®) we calculate current sequencing cost (chemistry plus personnel, but excluding the bioinformatic analysis) at roughly 150 200 Euro per sample, which may already now be interesting and feasible for routine screening purposes.
As the quantity of exogenous genes and GM crop events increase, several multiplex methods for simultaneous detection of more than one genetic element have been developed, such as multiplex PCR, microarray analysis, suspension array analysis, microdroplet-based PCR and others [ 4- 6].
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MARQUIS: a multiplex method for absolute quantification of peptides and posttranslational modifications.
However, there is no existing multiplex method for simultaneous HIV and HCV detection that is currently available.
Concentrations of inflammatory mediators (cytokines and chemokines) were measured using Miliplex® technology (Merck, Genese Diagnostics, Darmstadt, Germany), a multiplex method for cytokine analysis.
We measured a panel of 15 inflammatory mediators (cytokines and chemokines) using Miliplex® technology (Merck, Genese diagnostics, Darmstadt, Germany), a multiplex method for cytokine analysis.
AluScan implemented with just a small number of PCR primers based on consensus Alu sequences provides a multiplex method for genome-wide sequence analysis.
Thus, the results demonstrated that the nanobiochip sensor served as a specific and multiplex method for the determination of multiple salivary biomarkers from saliva and serum with sensitive signals and low DL within a short time [ 161].
This is one of the main aims of the EU integrated project Co-Extra: the development of a multiplex method for GMO analysis that can screen an individual sample for the presence of a large number of GMO varieties and GMO elements in a single analysis.
Using the multiplex method for cytokine analysis of supernatants taken after 96 hours of T-cell culture, we were not able to detect peptide-specific cytokine production (all mean values of IL-1α, IL-1β, IL-5, IL-10, IL-12, IL-18, TNF-α, or IFN-γ less than 2.0 pg/ml in patients with JIA and healthy controls).
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