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That system might be defensible were it used merely to inform parents, but for a multiplex manager to rule on what someone else's children may or may not see is impertinent.
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We used Multiplex Manager (Holleley and Geerts, 2009) to design and optimize multiplex PCRs to find annealing temperatures for each multiplex group to ensure specific amplifications and avoid complementary sequences among primers.
They were combined in two multiplexed reactions (Table 1) using Multiplex Manager 1.0 software (Holleley and Geerts, 2009).
The 14 polymorphic loci were combined in three multiplexed reactions (Table 1) using Multiplex Manager 1.0 software (Holleley and Geerts, 2009).
Multiplex Manager 1.2 software (Holleley and Geerts, 2009) was used to optimize primer combinations.
Multiplex panels were designed using Multiplex Manager [ 29].
Using Multiplex Manager version 1.2 (Holleley and Geerts, 2009), initial test multiplexes were developed and new forward primers were synthesized.
Multiplex PCR containing six (LML11) and seven (LMK13) microsatellites were set up, taking care to avoid complementary primers and markers with identical dye and amplicon size in the same multiplex with the help of Multiplex Manager (Holleley and Geerts, 2009).
Primers for the microsatellites were taken from Wright et al. (2004) and had been arranged into multiplexes with the help of Multiplex Manager 1.0 (Holleley and Geerts, 2009).
Seventeen polymorphic markers were selected for further analysis and combined into four multiplex PCRs with Multiplex Manager version 1.0 (Holleley and Geerts, 2009; PCR multiplex sets 1 4 in Table 2).
Using the software Multiplex Manager version 1.2 (Holleley and Geerts, 2009), two 6-plex and one 5-plex multiplexes were designed, and new fluorescently labeled forward primers synthesized (Invitrogen; Table 2).
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