Recovered strains were tested with a commercial multiplex line probe assay (Inno-LiPA Mycobacteria v2, Innogenetics, Ghent, Belgium) specific for MAC and 16 other different mycobacterial species (11 ).
The hybridization test performed with the multiplex line probe assay was negative apart from the genus-specific line probe.
The commercial GenoType Mycobacterium CM multiplex line probe assay (Hain Lifescience, Nehren, Germany) identified the organism as Mycobacterium abscessus.
The multiplex line in Figure 3 shows a clear increase in TPR as replication increases despite the loss of detection power that decreasing sequencing depth induces.
The multiplex line strongly favours adding more biological replicates despite the inherent loss of sequencing depth as shown by its dramatic positive slope for the TPR while maintaining a roughly constant, low FPR.
To determine whether such a variant was found within each of our multiplex lines, we first functionally annotated variants for each line using SeqAnt (Shetty et al. 2010).
To examine the utility of our device, multiplex and in-line negative selections were performed on two human heat shock factor (hHSF) proteins, hHSF1 and hHSF2.
Advances in multi-analyte technologies such as line assays, multiplex systems and micro-arrays allow for the development of sophisticated profile assays containing multiple different antigens.
Without all of the hoopla surrounding a hit at the multiplex — the lines, the marquees, the buzz in the corridors afterward — movies start to feel an awful lot like their more commodified cousin, television.
On June 19 it announced the introduction of its new Inverse Multiplexing (IMUX) capability, which will support additional lines and deliver bandwidth services up to 12 Mbps.
