Sentence examples for multiplex level from inspiring English sources

ALM ASA is a potential method for SNP typing at an ultra-low cost because of a high multiplex level and a simple optimization step for PCR.

An improved approach for increasing the multiplex level of single nucleotide polymorphism (SNP) typing by adapter ligation-mediated allele-specific amplification (ALM ASA) has been developed.

Unfortunately the coverage drops dramatically if the multiplex level is increased.

Genotyping was carried out at a multiplex level of four SNPs per well and data quality was assessed by duplicate DNA (n = 4).

Genotyping was performed at a multiplex level of using the Illumina Golden Gate genotyping system [ 19] and data quality was assessed by duplicate DNAs (n = 10).

The key objectives we consider in this paper include the number of SNPs per tube (multiplex level) and the percentage of SNPs assigned to full tubes (coverage).

The SNPs were genotyped at a multiplex level using Illumina's Golden Gate genotyping system (Oliphant et al. 2002), and data quality was assessed using duplicate DNA (n = 10).

Indeed, the MLPA assay of Bergval et al. [ 5] included 47 informative probe pairs (high multiplex level) and hybridisation occurred at a concentration of 0.25 nM of each probe, whereas a concentration of 5 nM of each probe gave high signal-to-noise ratios for positive results in our initial MOL-PCR tests with 6 probe pairs (low multiplex level) (data not shown).

And newly available sequencing platforms with up to 10 Gb output per lane and techniques for higher multiplex levels will make this method even faster and more cost-effective.

Such similar success rate indicates that higher multiplex levels of GoldenGate arrays do not affect the assay's effectiveness.

Achieving broad SNP coverage rapidly transitions from being very easy to very hard as the target multiplexing level (# of primer pairs per tube) increases.