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In addition, the clinical feasibility of the present novel multiplex kit was demonstrated.
A SNaPshot Multiplex Kit was used to analyze the gene polymorphisms (Genesky Biotechnologies, Inc., Shanghai, China).
An iTRAQ Reagent-4plex Multiplex kit was obtained from Applied Biosystems (Foster City, CA, USA).
Analysis of the performance panel revealed that the Hi3-1 multiplex kit was similar in sensitivity to the Architect system.
The sensitivity of the Hi3-1 Multiplex kit was assessed using four different commercially available performance panels (Table 3).
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The validation results demonstrate that the 24-locus multiplex kit is a robust and reliable identification assay as required for forensic DNA typing and databasing.
A set of 4 miRNA markers (miR214 as menstrual blood marker, miR451a as venous blood marker, miR888 and miR891a as semen markers) and a DNA STR multiplex kit were co-analyzed by capillary electrophoresis.
In addition, the Hi3-1 Multiplex kit is capable of detecting HIV/HCV coinfection, as assessed using samples from panel 3.
Microsatellite markers were amplified using polymerase chain reaction (PCR) in a 10 μl reaction using a multiplex PCR kit (Qiagen Inc .. PCR conditions for each reaction using the multiplex kit were 5 μl of Qiagen Master Mix (containing HotStarTaq DNA polymerase, dNTPs and 3 mM MgCl2), 0.625 μM of each primer, and 3 μl of genomic DNA.
We conclude that analyses of the miniY-STR quadruplex systems in addition to the commercial available Y-STR multiplex kits are highly useful for forensic practices of degraded DNA samples.
Fluorokine MAP cytokine multiplex kits were purchased from R&D Systems (Minneapolis, MN) and the procedures were conducted as suggested by the manufacturer.
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