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We demonstrate the portability of this technology by multiplex gene silencing, adopting different DNA binding platforms and interrogating thousands of genomic loci in different cell types, including primary T lymphocytes.
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Mechanism of Polycomb group gene silencing.
RNA interference (RNAi) is useful for selective gene silencing.
G1-CBT1.9 alshowsows efficient gene silencing in vivo.
Some of these problems are caused by gene silencing at the level of chromatin – so-called epigenetic gene silencing.
The resulting shRNA expression system displays robust, Sox9-dependent gene silencing.
MicroRNAs (miRNAs) are key regulators of sequence-specific gene silencing.
The method described here allows the quantitative analysis of gene silencing and may also facilitate function discovery of novel plant genes based on siRNA-mediated gene silencing.
In normal cells, it assures the proper regulation of gene expression and stable gene silencing.
RNAi-induced gene silencing controls gene expression at all levels, including transcription, mRNA stability and translation.
virus-induced gene silencing.
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