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GeXP employs eXpress Profiling (XP-PCR), a patented technology for multiplex gene expression profiling analysis by which up to 30 genes can be easily multiplexed in the same reaction.
In order to study multiplex gene expression in the premigratory neural crest, we used midbrain cross sections from avian dorsal neural tubes (Fig. 1a).
Combining multiplex gene expression with spatial information provides intriguing biological insight about distinct stem cell niches in the dorsal neural tube.
These results demonstrate that multiplex gene expression analysis in single cells is a powerful tool for examining the unique behaviors of individual embryo- or mESC-derived cardiac cells.
To further understand the anti-tumorigenic mechanisms of Tg6F, we performed a cancer pathway gene array analysis (Nanostring Technologies) using multiplex gene expression analysis with 770 genes from 13 cancer-associated canonical pathways.
This Spatial Genomic Analysis toolkit provides a straightforward approach to study quantitative multiplex gene expression in numerous biological systems, while offering insights into gene regulatory networks via synexpression analysis.
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To circumvent these problems and enable analysis of subpopulations within the neural crest at the single cell level in vivo in the avian neural tube, we present an imaging based Spatial Genomic Analysis (SGA) pipeline, which utilizes multiplex analysis of gene expression at single cell resolution in a manner that preserves spatial information.
Biomarkers related to OA (NO, PGE2, cytokines, chemokines, metalloproteinases) were measured by multiplex ELISA and gene expression analysis in chondrocytes.
Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2 were measured by multiplex analysis and gene expression was quantified by RT-PCR.
Investigators trying to develop assays for the accurate multiplex quantification of gene expression in small samples face many technical hurdles [ 5].
Beyond the details of our particular experimental model, the achievement of reliably quantitative multiplex measurements of gene expression in single cells is meaningful for a wide variety of applications.
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