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This tool takes advantage of yeast homologous recombination to build multiplex gene assembly and plasmid construction without restriction enzyme cloning, and can be used to randomize genes and promoters.
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Using this technology, DNA oligo synthesis and gene assembly steps occur right on silicon.
Therefore, the introduction of pseudo-backcrossing could benefit multiplex gene pyramiding.
Using three 10-gene panels designed to measure proinflammatory and apoptosis responses, we demonstrated sensitive and specific multiplex gene expression profiling directly from cell lysates.
* Incomplete gene assembly, tentative gene designation.
Our strategy for vector construction is modular, efficient, and expandable for multiplex gene editing.
Involvement of selected genes in the oxidative stress-mediated signaling pathway was explored using multiplex gene expression analysis.
Also, evaluation of the transcriptional regulation of antioxidant and apoptotic genes was carried out using Multiplex Gene Expression System.
These results indicate that the RDB assay could be used to detect multiplex target genes of GMCs rapidly and inexpensively.
For instance, multiplex identifier, target gene, target subfragment are missing from the representations from OBI.
Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance.
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