Sentence examples for multiplex fish from inspiring English sources
This comprises a simplified 'single denaturation mixed hybridization' procedure that combines multi-color karyotyping by Multiplex FISH (M-FISH), for simultaneous and unambiguous identification of all chromosomes at once, and the use of a Quantum Dot (QD) conjugate for the transgene detection.
We describe a simple "single denaturation mixed hybridization" procedure combining multi-color karyotyping by Multiplex FISH (M-FISH), for simultaneous and unambiguous identification of all chromosomes at once, and the use of a Quantum Dot (QD) conjugate for fast chromosomal assignment of transgenic insertions at the single cell level in mouse and rat models.
In the present report we describe a technical development whereby even faster chromosomal assignment of transgenic insertions at the single cell level in mouse and rat can be obtained by an efficient, combined hybridization procedure for Multiplex FISH (M-FISH) and the detection of transgene insertions, the latter of which are easily labeled with ultra-bright Quantum Dot (QD) conjugates.
For practical application, we provide proven multiplex FISH protocols for AP- and PO-based visualization of mRNAs at high resolution.
This work presents the first study that develops, optimizes and validates a multiplex FISH procedure using locked nucleic acid (LNA) and 2′-O-methyl RNA (2′OMe) oligonucleotides probes for the in vitro discrimination within mixed populations.
Four color images using differently labeled PNA probes showed simultaneous identification of E. coli, P. aeruginosa, S. aureus and Salmonella, thereby demonstrating the potential of multiplex FISH for various diagnostic applications within both clinical and industrial microbiology.
The progress of FISH technologies based on chromosome painting, SKY and multiplex-FISH or multicolour-FISH (M-FISH) (Schröck et al, 1996; Speicher et al, 1996) has overcome the limitations of conventional cytogenetic methods in the characterisation of complex chromosome alterations observed in cancers.
Multiplex (M -FISH revealed a translocation t(11; 11) in 24% and a translocation t(11; autosoM -FISH5% of analyzed metaphases (Additional file 13C).
Briefly, the technique consists of co-hybridizing the directly labeled M-FISH multiplex probe for the animal species under investigation together and the indirectly labeled transgenic probe to the chromosome preparation, previously fixed on slide.
Each of the chromosome-specific "paints" in the commercially obtainable multiplex or M-FISH probe is labeled with a finite number (five in our case) of distinct organic fluorophores (spectrally compatible to DEAC, FITC, Spectrum Orange™, Texas Red® and Cy™5) in a unique combinatorial fashion, while the single transgenic probe is labeled in-house with a reporter molecule (Biotin in our case).
Of the 13 CLL patients included in this study, 7 (54%) had a deletion at 13q14 confirmed by fluorescence in situ hybridisation (FISH) and multiplex ligation-dependent probe amplification (MLPA) (data not shown).
