Sentence examples for multiplex experiment from inspiring English sources

Tests on pure culture smears showed that this method is able to distinguish 3 different microorganisms with distinct cell wall characteristics in a multiplex experiment.

Because the intention was to form mono-, duand and tri-species biofilms, both probes would have to be specific to the species of interest at the same hybridization and washing temperature in a multiplex experiment.

While detecting both Gram- and Gram+ microorganisms using the same fixation/permeabilization method has been proved difficult for conventional DNA probes [23], the particular characteristics of PNA (such as the uncharged backbone), had already allowed to develop a multiplex experiment for both groups of microorganisms as early as 2001 [44].

We designed a multiplex experiment where the volumes of chromatin and washings were reduced to 150 µl, which allows the use of standard PCR strips and multichannel pipettes.

The substantial underrepresentation of samples indexed with BC4 has also been revealed in other in-house multiplex experiment including BC4 on human gDNA samples using different sample enrichment technologies (see Discussion).

A typical three-fold multiplex experiment with cDNA templates is shown in Figure 2. Three pairs of PCR primers were combined in a single PCR reaction for each of the CMMB cDNA templates (Fig. 2A).

Specific probes for these species were designed and optimized for specific hybridization in multiplex experiments.

Results showed that the LNA/2′OMe-FISH method performed well in multiplex experiments and presented a good correlation with total and cultivability counts, regardless of the cells physiological state.

In addition to the complexity of designing and performing highly multiplex experiments, the cost of reagents and the time of protocol executions often limit the experiments.

The help of Mr Keith Fourrier with the multiplex experiments and data analysis is gratefully acknowledged.

Multiplex experiments were performed using the QuantiTect Multiplex PCR Kit (Qiagen) with an ABI 7900HT instrument (Applied Biosystems).