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Among others, Dinish et al. reported the in vivo triplex detection of cancer markers in xenograft breast cancer model (Dinish et al. 2014), even though the largest number of multiplex discrimination of SEPs in vivo was demonstrated for ten different nanoconstructs nonspecifically accumulated in the liver of a mouse (Zavaleta et al. 2009).
We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples.
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Multiplex gas discrimination for room temperature can be achieved by strategically choosing the right metal/CNT combination in a complete sensor system.
Compared to the Yfiler PCR Amplification Kit, the new multiplex shows increased discrimination of male lineages and also improved performance in inhibited samples, improved balance in male DNA samples mixed with female DNA at ratios >1 1000, and faster time to results.
In this study, we developed a simple multiplex-PCR system, named MTC-discrimination multiplex PCR (MTCD-MPCR), to distinguish M. tuberculosis from other MTC species using RDs, and applied it for clinical isolates derived from TB patients in Bangladesh.
The MTC-discrimination multiplex PCR (MTCD-MPCR) developed in this study showed high specificity and was thought to be very useful as a routine test because of its simplicity.
In the current study, the MTC-discrimination multiplex PCR (MTCD-MPCR) was developed and applied for a study to see the prevalence of MTC species other than M. tuberculosis in clinical isolates in Bangladesh.
Two primers are used to amplify samples and a third unique indexing primer containing the indexing barcode is used to for samples discrimination after multiplex sequencing.
We screened for 38 known BRCA1 mutations using allelic discrimination and multiplex PCR accounting for 853 of 1166 BRCA1 families (∼73%) in the Netherlands (Supplemental Table S1, available at Annals of Oncology online) (M. K. Schmidt et al., unpublished data).
Future studies should enable us to develop an assay specifically for S. pseudopneumoniae and a real-time, multiplex assay for rapid discrimination of the most important viridans streptococci in bacterial cultures or patient samples.
By using the three new sets of ORF-0 specific primers and CP+/CP− primers in a single multiplex RT-PCR, the detection and discrimination of the three beet Polerovirus species was possible in infected plants.
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