The PowerPlex Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.
The PowerPlex®Y23 System (PPY23, Promega Corporation, Madison, WI) is a five-dye Y-STR multiplex designed for genotyping male samples at 23 loci.
The use of a multiplex design allows for simultaneous testing for numerous species, reducing processing and handling times, lowering costs and reducing the amount of DNA extract required for testing.
Secondly it is possible to apply dedicated AIM-SNP multiplexes designed for specific population comparisons as described above.
However, the loci searched can be of immense use as it has location and chromosome, which is required input for multiplex designing.
Using multiplex primers designed for detection of E. coli cdt genes, PCR analysis indicated the presence of cdt genes in 37.5% of these isolates.
Multiplex PCR designed for genotyping the genus Trichinella revealed that the Sapporo isolate showed a specific pattern to T. britovi complex (T. britovi, Trichinella T8 and Trichinella T9) and the Otofuke isolate showed that to T. nativa.
Here, we describe the application of a multiplex PCR (mPCR) design for simultaneous detection of four virulence genes (set1A, set1B, ial and ipaH) in Shigella spp. and to determine the prevalence of these virulence genes in a random selection of Malaysian Shigella strains.
Two novel multiplex assays were created with the endpoint PCR primers: multiplex 1 amplifies two markers for each of the above mentioned body fluids and is suited for screening; multiplex 2 was designed for the detection of blood, vaginal secretions and menstrual blood.
We report the development of a qualitative fluorescent multiplex homogeneous assay designed for the detection of the two most common hemochromatosis mutations using dual-labeled fluorescent probes.
