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Due to the multiplex design, automation, speed of analysis, and ability to interrogate mixtures, this method provides a powerful and rapid tool for forensic mtDNA examinations.
The use of a multiplex design allows for simultaneous testing for numerous species, reducing processing and handling times, lowering costs and reducing the amount of DNA extract required for testing.
Allele-specific primers tailed with shared universal tag sequences were designed to speed multiplex design and balance the amplification efficiencies of all loci through the use of a single reverse and two differentially labelled allele denoting forward universal primers.
Due to the multiplex design of the amplification, the RT reaction is divided into multiple PCR reactions.
Multiplex design for Sequenome® MassARRAY® iPLEX Gold platform included 59 out of 60 selected SNPs divided into three multiplexes containing 32, 24 and 3 SNPs, respectively.
The initial multiplex design identified the serotype of 31% (N = 39/125) and 48% (N = 64/132) of pneumonia/sepsis and meningitis isolates, respectively (p = 0.007).
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This technique is the first reported real-time PCR method for BPIV-3 and the first multiplex designed to detect three BRDC viral pathogens.
The PowerPlex Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.
Such multiplex designing needs much more number of markers to design multiplex with thermodynamic compatibility, which can be accomplished from our large marker dataset.
However, the loci searched can be of immense use as it has location and chromosome, which is required input for multiplex designing.
The PowerPlex®Y23 System (PPY23, Promega Corporation, Madison, WI) is a five-dye Y-STR multiplex designed for genotyping male samples at 23 loci.
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