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After genotyping with the first multiplex combination of five loci the family affiliations were established for a total of 1, 910 individuals (97%).
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In addition, we identified multiplex combinations of microsatellite loci that facilitate rapid genome-wide genotyping and demonstrate the utility of these microsatellite loci in a preliminary investigation of Ae. aegypti population genetic structure in Haiti.
First, we describe the cellular diversity within the VTA, including neurons capable of transmitting dopamine, glutamate or GABA as well as neurons capable of multiplexing combinations of these neurotransmitters.
Three pairs of primer sequences displayed some degree of similarity and should be avoided as potential multiplex combinations to prevent the risk of forming primer dimers (CAM-02R CAM-15R CAM-02R CAM-15R CAM-02R CAM-15RM-06R).
Upon adjustment of primers and buffer concentrations, specific and consistent amplification of all the genes in the multiplex combination was achieved.
First, we selected the best multiplex combination using all 232 SNPs.
The 24 SSRs in the six multiplex combinations yielded a total of 159 alleles.
Multiplex combinations were compared with single PCRs in presence of the internal control Phocine Herpes Virus (PhHV).
Primers were grouped into multiplex combinations shown in Table S4.
Three post-PCR multiplex combinations (Table 1) were designed by considering the fragment size and dye.
To achieve higher order multiplexing, combination with multiplexed primer should be introduced in the future.
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