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The ability to express two or more gRNAs within the same cell may permit multiplex cleavage events that can generate large deletions, which could be used to investigate genome duplications that are often associated with drug resistance.
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Such utilities include multiplexing for inducing multiple cleavage events, controlling gene expression, and site specific transgene insertion.
During the maturation process, multiple cleavage events including CA-SP1 sequentially take place.
These cleavage events produce intermediate as well as final cleavage products that together with host factors initiate replication complex formation.
This process includes cleavage at an internal Arg-Val site to form an N-terminal A-chain and C-terminal B-chain held together by a disulfide bond, and can include further proteolytic cleavage events to remove the preactivation peptide.
The first two cleavage events leave the resulting RNA with a 5′-OH group and a 2′,3′-cyclic phosphate.
Although this cleavage should happen specifically at the enzyme's recognition sequence, an unknown proportion of cleavage events may involve other sequences, owing to the enzyme's star activity or to random DNA breakage.
Pattern analysis of cleavage events indicated putative targeting sequences during the GV-MII transition.
Two LSU rRNA fragments appear first, followed by smaller fragments produced by additional cleavage events.
No such cleavage events were observed in 8226 GFP cells.
Ribosome biogenesis involves a number of pre-rRNA cleavage events.
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