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The cLPP multiplex capture of thousands of exons in a single tube can be performed within approximately 4 hours.
In the analysis of data from the multiplex capture of common coding SNPs using padlock probes (see below), we restricted the analysis to SNPs with 100 reads or more.
In order to build a cost-effective but comprehensive diagnostic approach, we performed multiplex capture of the regions of interest using patients' DNA followed by sequencing with an Illumina Genome Analyzer.
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The key innovation of our method is multiplex capture and sequence that allows the simultaneous survey of both mutant, parental, and background strains in a single experiment.
Comparison of different multiplexed capture pools demonstrates high capture efficiency and genome coverage of B. burgdorferi even when multiplexing up to 20 genomic libraries in a single capture reaction (Tables 1 and 2), with equivalent sensitivity in SNP detection observed in the pairwise comparison of the replicates in the 10-plex or 20-plex reaction (Additional file 1: Table S4).
We also enforce different configurations of the multiplex to capture the heterogeneity observed in the real networks of buses and metros as shown in Fig. 3.
The specific capture of TUNEL-labeled fragment (α and β) was assessed by multiplex PCR amplification (Qiagen Multiplex PCR Kit, Qiagen Inc).
Couple all that with the shocking Taliban capture of a key Afghan regional capital and America's multiplex strategic failures in the Middle East, North Africa, and Central Asia begin to come into focus.
For example, a multiplex assay of anti-EpCAM and anti-MUC1 modified MNPs increased the capture of the low EpCAM expressing cell line BT-20 from the 45% yield achieved with anti-EpCAM alone to 78%.
"The capture of Noriega.
Following QIAquick purification, the pool of cLPPs is ready for multiplex target capture.
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