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Leveraging the multiplex capability of the Cpf1 platform, we demonstrate both synergistic and combinatorial gene expression in human cells.
The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer.
The miniaturized integrated CMOS microchip system with CAE has excellent advantages over conventional instrumental systems for analysis of bacterial pathogens such as compactness, low cost, high speed, and multiplex capability.
This multiplex capability of the platform makes it a good tool for complex phenotyping in large scale toxicology.
To demonstrate the multiplex capability of the cell chip, we printed chips with both A549 non-small-cell lung cancer cells and HeLa cervical cancer cells.
However, EDC fixation may limit the multiplex capability of miRNA and protein co-detection because EDC cross-links can cause epitope masking [ 79].
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Utilizing massively parallel sequencing (MPS) technology for SNP testing in forensic genetics is becoming attractive because of the shortcomings of STR markers, such as their high mutation rates and disadvantages associated with the current PCR-CE method as well as its limitations regarding multiplex capabilities.
Because of the public health importance of technologies with multiplex capabilities, this criterion was allotted two hundred points.
This approach can provide objective quantification and multiplex capabilities with high sensitivity and in an antibody-free setting [ 78- 80].
The assay combines the multiplex capabilities and specificity of ligation reactions with high-throughput real-time PCR in the OpenArray™, resulting in a flexible, quantitative multiplex diagnostic system.
These include the need for high levels of sensitivity as well as specificity, coupled with quantitative, multiplex capabilities and high sample throughput.
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