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Multiplex assays to identify the presence of a wide variety of microbes within a specimen will have a profound impact on the efficient management of disease treatments and prevention, clinical follow-up studies, and the development of new therapies of prophylactic or therapeutic vaccines.
Other investigators have used antibody-based multiplex assays to characterize microbial pathogens and agents [ 42– 42].
We designed two multiplex assays to genotype all 29 SNPs using Assay Design software (Sequenom, San Diego, CA, USA).
However, once validation strategies have proved multiplex assays to be as robust, reliable and reproducible as single-plex assays, they are destined to comprise a significant part of clinical trial activities.
To determine whether the automated IMPACT platform could recapitulate this finding, we used the IMPACT platform in conjunction with bead-based multiplex assays to characterize serum samples from 120 RA patients, of which 73 had anti-CCP antibodies (as assessed by the IMPACT assay), 78 had RF (as assessed by the IMPACT assay), and 74 had one or two SE alleles.
For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays.
Similar(49)
The objective of the study was the evaluation of a novel multiplex assay to detect fetal Rh blood group D-antigen gene (RHD) loci in maternal plasma from RhD-negative, pregnant women.
A workable combined assay (e.g. a multiplex assay) to determine these antibodies is still not available.
We used a multiplex assay to measure levels of 25 cytokines and chemokines in plasma samples from 20 AIDS patients in the study cohort and explore their relationship to individual IBD serological markers.
We developed a novel multiplex assay to detect norovirus (types 1 and 2), astrovirus, and sapovirus.
Other genes could be used in this multiplex assay to address complementary, but different, questions.
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