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Study design: The multiplex assay was designed using Access RT-PCR (Promega).

The multiplex assay was verified testing genomic DNA of 24 carbapenemase-producing strains.

The multiplex assay was tested against 50 isolates and 26 apothecial field samples of H. albidus/H.

Multiplex assay was validated through comparison of IgG levels to 14 randomly chosen pneumococcal antigens by using multiplex and singleplex assays.

The multiplex assay was compared successfully to the single SYBR Green assay, and revealed to be at least 10 times more sensitive than the one obtained with an endpoint PCR thermocycler protocol published previously.

Compared to phenotypic testing (E-test or CDT), the TAT for the combined multiplex assay was short (~5 h).

Median Fluorescent Intensity; The Lyme multiplex assay is based on fluorescent detection technology.

The sensitivity, specificity and potential applications of the multiplex assay are discussed.

The individual RT-PCR assays and the combined multiplex assay were optimized for highest sensitivity and specificity.

Typically, one or two antibody values (out of the three Osp values in the multiplex assay) are increased more than 2-fold if B. burgdorferi contributes to the neurologic condition.

All the autosomal SNPs (from the SNPforID Consortium) included in the multiplex assay are unlinked and are distributed widely across autosomes, enabling genetic fingerprints to be distinguished.