We evaluated the MEBGENTM RASKET KIT (RASKET KIT), a multiplex assay using PCR-reverse sequence specific oligonucleotide and xMAP® technology to concurrently detect exon 2, 3, and 4 RAS mutations in a short turnaround time (4.5 h/96-specimens).
Angiopoietin-1, angiopoietin-2, protein C, antithrombin, matrix metalloproteinase (MMP -8, tissue inhibitor of MMP -8oprotissuee (Tinhibitor&D systems, Abingdof, UK), and D-dimetalloproteinase, eBioscience, San Diego, CA) were measured by Luminex mulTIMP -1assay using a BioPlex 200 (BioRad, Hercules, CA).
Recently, a small proof-of-concept study assessed the multiplex assay using DBS samples from QFT-TB stimulated blood [ 32].
Five nucleotide variations (nt289, nt793, nt1199, nt1385, nt1623) were genotyped simultaneously in a multiplex assay using oligonucleotides distinguishable by length.
IL-6 levels in plasma from subjects injected with LPS were determined by Luminex multiplex assay using BioPlex 200 (BioRad, Hercules, CA, USA).
The sensitivity of the assay was evaluated individually for each virus in the multiplex assay using serial ten-fold dilutions of cloned PCR products.
However, neutrophil migration might be influenced by other chemokines that were not analysed with the multiplex assay used.
The multiplex assay used a published primer set (CRY1 and CRY2) for detecting A. cryaerophilus DNA (Houf, K., Tutenel, A., De Zutter, L., Van Hoof, J. and Vandamme, P., 2000.
The multiplex assay used Beadmate kits (Upstate) as specified in the manufacturer's instructions, read with a Luminex 100 system (Upstate).
However, this study differed from the present study with regard to study design, mouse strain and the type of multiplex assay used.
The multiplex assay used Q-Plex™ technology (Quansys BioScience, Logan, UT), which involves the micro-spotting of individual groups of capture antibody in either a cartesian or polar coordinate system on the bottom of a 96 well plate with each spot being its own 'micro ELISA' assay [ 9].
