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HIrisPlex consists of a single multiplex assay targeting 24 eye and hair colour predictive DNA variants including all 6 IrisPlex SNPs, as well as two prediction models, a newly developed model for hair colour categories and shade, and the previously developed IrisPlex model for eye colour.
MLVA profiles were generated using a multiplex assay targeting five VNTR loci [ 69].
Nevertheless, the drawback linked to the PCR complicates the setting of a unique multiplex assay targeting simultaneously all GM events.
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The panel of singleplex reactions targeted seven viruses, while the multiplex assay targeted thirty-three bacteria, fungi, and viruses.
To facilitate their rapid identification, we developed a multiplex PCR assay targeting conserved, species-specific genes.
Therefore we developed a multiplex PCR assay targeting avrBsT and xopL for the molecular identification of Xap.
Hygromycin-resistant sugar beet roots were evaluated for the presence of the cassette and for absence of A. rhizogenes cells using a multiplex PCR assay targeting the nucleotide sequences of the hrpZPsph transgene and virCD of A. rhizogenes, using FTA (Whatman -immobilized nucleic acids as template for amplification.
For Rickettsia typhi and R. felis, a real-time multiplex PCR assay targeting a conserved fragment of gltA was used (unpub. data).
During January 1 , 2004 December 31 , 2013real-time PCR testing was performed on DNA extracted from EDTA whole blood samples for A. phagocytophilum, E. chaffeensis, E. ewingii, and the EML pathogen by using a modified version of a multiplex PCR assay targeting a conserved region of the groEL heat-shock protein operon (13 ) (Technical Appendix Table 1).
A tempting application of this method would be a multiplex modular domain assay targeting multiple PTMs on receptor tyrosine kinases such as EGFR or PDGFR.
Peripheral inflammation was monitored using a serum-based multiplex bead assay targeted for key cytokines, namely Interleukin (IL -1β, IL -1βnd tumor necrosis alpha (TNF-α).
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