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No TNFα was present, however, in the multiplex assay of cytokine-pretreated fibroblast supernatants (Table 2).
We have been developing the use of tandem mass spectrometry for the multiplex assay of several lysosomal enzymes in dried blood spots on newborn screening cards.
Isolates that were nontypeable or found to possess a SCC mec IV underwent further mec characterization according to the multiplex assay of Zhang et al [ 17].
In this molecular epidemiological surveillance, approximately 51% (2009/3935) of patients had at least one viral pathogen detected by the multiplex assay, of whom 0.6% (12/2009) were infected with EV-D68.
For example, a multiplex assay of anti-EpCAM and anti-MUC1 modified MNPs increased the capture of the low EpCAM expressing cell line BT-20 from the 45% yield achieved with anti-EpCAM alone to 78%.
We genotyped the CNV with a modified protocol from that used by Cid et al, 19 a multiplex assay of two fluorescently marked PCR products detected on an ABI3730 DNA sequencer (Applied Biosystems, Foster City, California, USA).
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Example (step 2) cycle and melt curves are shown in Figures 1, 2, 3, 4, after 15 pre-amplification cycles in Step 1. Step 2 cycling and melt curves in multiplex assays of gene targets specific for influenza A, influenza B, respiratory syncytial virus (RSV), rhinovirus (RV) and human para-influenza virus 3 (hPin3) in Figures 1-5.
The SNP marker has become the marker of choice for many research applications because of the abundance of SNPs and the several technologies available for the high-multiplex assay of SNPs [ 1].
We present the potential use of a planar method as multiplex assay for the identification of a large panel of inflammatory mediators in blood.
To our knowledge, no previous study has reported the development of a DPO multiplex assay for the simultaneous detection of influenza A, B and subtypes of influenza A including H5N1.
A one-step multiplex assay for the detection of the specific serotype of DENV was carried out according to the protocol described by Johnson et al. (12).
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