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A selection of the 23 cytokines detected by the multiplex assay is shown in Figure 4.
In addition, the identification of several GM targets using a multiplex assay is not applicable [ 28].
Compared to the electrophoresis gel, the resolution power of the CGE system to detect PCR products from a multiplex assay is clearly higher [ 134].
An example of such a multiplex assay is microarray surface plasmon resonance imaging (iSPR), which is a label-free method used to detect biomolecular interactions in real time.
Our novel multiplex assay is an accurate and reliable method which can be used in place of the conventional method for S. pneumoniae carriage studies.
This multiplex assay is composed of 7 consecutive PCR reactions combining primers that have been already described in different studies [ 23, 25, 26] (Table 3).
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Study design: The multiplex assay was designed using Access RT-PCR (Promega).
The sensitivity, specificity and potential applications of the multiplex assay are discussed.
The multiplex assay was verified testing genomic DNA of 24 carbapenemase-producing strains.
Compared to phenotypic testing (E-test or CDT), the TAT for the combined multiplex assay was short (~5 h).
The individual RT-PCR assays and the combined multiplex assay were optimized for highest sensitivity and specificity.
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