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The reproducibility of the bead-based multiplex array was determined by establishing inter-assay and intra-assay coefficients of variance (CVs).
Within the anti-CCP-2-negative subset, positivity in the multiplex array was not associated with progression (30%% in multiplex-negative versus 30%% in multiplex-positive).
Within anti-CCP-2-negative patients, ACPA reactivity as detected by multiplex array was not significantly associated with known risk factors or clinical or prognostic parameters.
To search for other potentially important cell recruitment mediators, a multiplex array was performed on a subset of end-stage lung disease patients' plasma.
Through combinatorial analysis of all 3 biomarkers using optimal cutoff values defined by Youden index calculations, the AUROC for the diagnostic panel using the multiplex array was 0.9476 [95% CI: 0.903 - 0.992].
Similar(55)
The advantage but also challenge of a multiplex array is the combination of several assays on a single platform.
A major risk in the multiplex arrays is the increased cross-reactivity, due to the presence of multiple antibodies, which are normally applied as a mixture.
Recently, a multiplex protein array was also employed, providing high-throughput analysis [ 61]; however, this method requires some prior knowledge of likely analytes.
A novel multiplexed ratiometric biosensor array was fabricated on a homemade screen-printed carbon electrode (SPCE) for near-simultaneous detection of microRNA (miRNA -21 and miRNA -21 bandd on electrochemiluminescence (ECL) coupled with cyclic voltammetry (CV) method.
Multiplex bead array is compared to the "gold standard," ELISA.
Performance of the multiplex pan FMDV (MpFMDV) array was compared to the 5′ UTR and two 3Dpol assays, following tests on the Miniopticon (BioRad) and 7900HT SDS (ABI) platforms.
Related(16)
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